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recombinant human trail protein  (R&D Systems)


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    Structured Review

    R&D Systems recombinant human trail protein
    Recombinant Human Trail Protein, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 132 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+human+trail/Recombinant+Human+TRAIL%2FTNFSF10+Protein/pm41779305-92-0-5
    Average 94 stars, based on 132 article reviews
    recombinant human trail protein - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Recombinant:

    Article Title: Chaetocin enhances tumor necrosis factor‑related apoptosis‑inducing ligand‑mediated apoptosis by enhancing DR5 stabilization and reactive oxygen species generation in human glioblastoma cells.
    Article Snippet: Chaetocin, a fungal metabolite, exerts notable antiproliferative effects against solid tumors by triggering apoptosis; however, the mechanisms underlying its effects remain unclear.. As tumor necrosis factor (TNF)‐related apoptosis‐inducing ligand (TRAIL) promotes apoptosis in certain types of tumor, the present study aimed to explore the sensitizing effects of chaetocin in TRAIL‐induced apoptosis in human glioblastoma cells and the underlying mechanism.. Human glioblastoma cells (U343MG, U87MG, U251MG, and T98G) and embryonic kidney cells (HEK293) were co‐treated with chaetocin and TRAIL, followed by assessment of cell viability.

    Article Title: Metformin induces apoptosis in TRAIL-resistant colorectal cancer cells.
    Article Snippet: .. Cells were incubated at 37 ◦C with 5 % CO2 in a humidified incubator until 80 % confluence and then treated with metformin (Sigma-Aldrich, St. Louis, MO, USA) and/or recombinant human TRAIL (R&D systems, Minneapolis, MN, USA). ..

    Article Title: The tetrapeptide sequence of IL-18 and IL-1β regulates their recruitment and activation by inflammatory caspases
    Article Snippet: Other reagents used include: LPS-EB Ultrapure (Invivogen, tlrl-3pelps), VX-765 (Apexbio Technology LLC, 50-101-3604), Z-VAD-FMK (Enzo Life Sciences, NC9471015), FuGENE HD (Promega, E2311), AP20187 (Tocris 6297/5), NP-40 Lysis Buffer Low Salt (Thomas Scientific, C994H79). .. Pam3CSK4 (Invivogen, tlrl-pms), phorbol 12-myristate 12-acetate (Promega, V1171), NP-40 Lysis Buffer Low Salt (Thomas Scientific, C994H79), Recombinant Human TRAIL (R&D Systems, 375-TL/CF) Staurosporine (MedChemExpress, HY-15141), Etoposide (Enzo Life Sciences, BMC-GR307), Nigericin (Cayman Chemical, 11437), Doxycycline hydrochloride (Fisher Scientific, ICN19504401), Cyquant LDH Cytotoxicity Assay (Thermo Scientific, C20300), DC Protein Assay Kit (Bio-Rad, 5000111), HEK-Blue IL-18 (Invivogen, hkb-hmil18), HEK-Blue IL-1b (Invivogen, hkb-il1bv2), Human Total IL-18/IL-1F4 Quantikine ELISA Kit (R&D Systems, DL180), Mouse IL-1 beta/IL-1F2 Quantikine ELISA Kit (R&D Systems, MLB00C), Recombinant Human Caspase-1 (Enzo Life Sciences, BML-SE168-5000), Recombinant Human Caspase-4 (Enzo Life Sciences, BML-SE176-5000), Recombinant Human Caspase-5 (Enzo Life Sciences, BML-SE171-5000), Recombinant Mouse Caspase-11 (Enzo Life Sciences, BML-SE155-5000). .. To quantify the amount of active IL-1β and IL-18 released, HEK-Blue IL-18 and HEK-Blue IL-1β (Invivogen) reporter cells were used according to manufacturer’s protocols, as previously described.

    Article Title: The tetrapeptide sequence of IL-18 and IL-1β regulates their recruitment and activation by inflammatory caspases.
    Article Snippet: Other reagents used include: LPS-EB Ultrapure (Invivogen, tlrl-3pelps), VX-765 (Apexbio Technology LLC, 50-101-3604), Z-VAD-FMK (Enzo Life Sciences, NC9471015), FuGENE HD (Promega, E2311), AP20187 (Tocris 6297/5), NP-40 Lysis Buffer Low Salt (Thomas Scientific, C994H79). .. Pam3CSK4 (Invivogen, tlrl-pms), phorbol 12-myristate 12-acetate (Promega, V1171), NP-40 Lysis Buffer Low Salt (Thomas Scientific, C994H79), Recombinant Human TRAIL (R&D Systems, 375-TL/CF) Staurosporine (MedChemExpress, HY-15141), Etoposide (Enzo Life Sciences, BMC-GR307), Nigericin (Cayman Chemical, 11437), Doxycycline hydrochloride (Fisher Scientific, ICN19504401), Cyquant LDH Cytotoxicity Assay (Thermo Scientific, C20300), DC Protein Assay Kit (Bio-Rad, 5000111), HEK-Blue IL-18 (Invivogen, hkb-hmil18), HEK-Blue IL-1b (Invivogen, hkbil1bv2), Human Total IL-18/IL-1F4 Quantikine ELISA Kit (R&D Systems, DL180), Mouse IL-1 beta/IL-1F2 Quantikine ELISA Kit (R&D Systems, MLB00C), Recombinant Human Caspase-1 (Enzo Life Sciences, BML-SE168-5000), Recombinant Human Caspase-4 (Enzo Life Sciences, BML-SE176-5000), Recombinant Human Caspase-5 (Enzo Life Sciences, BML-SE171-5000), Recombinant Mouse Caspase-11 (Enzo Life Sciences, BML-SE155-5000). .. HEK Blue IL-1R reporter assay To quantify the amount of active IL-1b and IL-18 released, HEK-Blue IL-18 and HEK-Blue IL-1b (Invivogen) reporter cells were used according to manufacturer’s protocols, as previously described.42 Briefly, binding of IL-18 to the IL-18R or IL-1b to the IL-1R induces NF-kB activation downstream, which leads to dose-dependent productions of secreted embryonic alkaline phosphatase (SEAP).

    Article Title: Chaetocin enhances tumor necrosis factor-related apoptosis-inducing ligand-mediated apoptosis by enhancing DR5 stabilization and reactive oxygen species generation in human glioblastoma cells
    Article Snippet: Chaetocin was obtained from Enzo Life Sciences, Inc. (cat. no. BML-GR349). .. Pan-caspase inhibitor z-VAD-fmk and recombinant human TRAIL were purchased from R&D Systems, Inc. (cat. nos. ..

    Article Title:
    Article Snippet: .. Recombinant human TRAIL was purchased from R&D System, Inc (Minneapolis, MN), and was used for cell stimulation in all experiments at a concentration of 20 ng/ml or as indicated in the figure legends. ..

    Article Title: The generation of stable microvessels in ischemia is mediated by endothelial cell derived TRAIL
    Article Snippet: .. Cells were treated with antibodies or recombinant human TRAIL (R&D Systems) as indicated. ..

    Incubation:

    Article Title: Metformin induces apoptosis in TRAIL-resistant colorectal cancer cells.
    Article Snippet: .. Cells were incubated at 37 ◦C with 5 % CO2 in a humidified incubator until 80 % confluence and then treated with metformin (Sigma-Aldrich, St. Louis, MO, USA) and/or recombinant human TRAIL (R&D systems, Minneapolis, MN, USA). ..

    Lysis:

    Article Title: The tetrapeptide sequence of IL-18 and IL-1β regulates their recruitment and activation by inflammatory caspases
    Article Snippet: Other reagents used include: LPS-EB Ultrapure (Invivogen, tlrl-3pelps), VX-765 (Apexbio Technology LLC, 50-101-3604), Z-VAD-FMK (Enzo Life Sciences, NC9471015), FuGENE HD (Promega, E2311), AP20187 (Tocris 6297/5), NP-40 Lysis Buffer Low Salt (Thomas Scientific, C994H79). .. Pam3CSK4 (Invivogen, tlrl-pms), phorbol 12-myristate 12-acetate (Promega, V1171), NP-40 Lysis Buffer Low Salt (Thomas Scientific, C994H79), Recombinant Human TRAIL (R&D Systems, 375-TL/CF) Staurosporine (MedChemExpress, HY-15141), Etoposide (Enzo Life Sciences, BMC-GR307), Nigericin (Cayman Chemical, 11437), Doxycycline hydrochloride (Fisher Scientific, ICN19504401), Cyquant LDH Cytotoxicity Assay (Thermo Scientific, C20300), DC Protein Assay Kit (Bio-Rad, 5000111), HEK-Blue IL-18 (Invivogen, hkb-hmil18), HEK-Blue IL-1b (Invivogen, hkb-il1bv2), Human Total IL-18/IL-1F4 Quantikine ELISA Kit (R&D Systems, DL180), Mouse IL-1 beta/IL-1F2 Quantikine ELISA Kit (R&D Systems, MLB00C), Recombinant Human Caspase-1 (Enzo Life Sciences, BML-SE168-5000), Recombinant Human Caspase-4 (Enzo Life Sciences, BML-SE176-5000), Recombinant Human Caspase-5 (Enzo Life Sciences, BML-SE171-5000), Recombinant Mouse Caspase-11 (Enzo Life Sciences, BML-SE155-5000). .. To quantify the amount of active IL-1β and IL-18 released, HEK-Blue IL-18 and HEK-Blue IL-1β (Invivogen) reporter cells were used according to manufacturer’s protocols, as previously described.

    Article Title: The tetrapeptide sequence of IL-18 and IL-1β regulates their recruitment and activation by inflammatory caspases.
    Article Snippet: Other reagents used include: LPS-EB Ultrapure (Invivogen, tlrl-3pelps), VX-765 (Apexbio Technology LLC, 50-101-3604), Z-VAD-FMK (Enzo Life Sciences, NC9471015), FuGENE HD (Promega, E2311), AP20187 (Tocris 6297/5), NP-40 Lysis Buffer Low Salt (Thomas Scientific, C994H79). .. Pam3CSK4 (Invivogen, tlrl-pms), phorbol 12-myristate 12-acetate (Promega, V1171), NP-40 Lysis Buffer Low Salt (Thomas Scientific, C994H79), Recombinant Human TRAIL (R&D Systems, 375-TL/CF) Staurosporine (MedChemExpress, HY-15141), Etoposide (Enzo Life Sciences, BMC-GR307), Nigericin (Cayman Chemical, 11437), Doxycycline hydrochloride (Fisher Scientific, ICN19504401), Cyquant LDH Cytotoxicity Assay (Thermo Scientific, C20300), DC Protein Assay Kit (Bio-Rad, 5000111), HEK-Blue IL-18 (Invivogen, hkb-hmil18), HEK-Blue IL-1b (Invivogen, hkbil1bv2), Human Total IL-18/IL-1F4 Quantikine ELISA Kit (R&D Systems, DL180), Mouse IL-1 beta/IL-1F2 Quantikine ELISA Kit (R&D Systems, MLB00C), Recombinant Human Caspase-1 (Enzo Life Sciences, BML-SE168-5000), Recombinant Human Caspase-4 (Enzo Life Sciences, BML-SE176-5000), Recombinant Human Caspase-5 (Enzo Life Sciences, BML-SE171-5000), Recombinant Mouse Caspase-11 (Enzo Life Sciences, BML-SE155-5000). .. HEK Blue IL-1R reporter assay To quantify the amount of active IL-1b and IL-18 released, HEK-Blue IL-18 and HEK-Blue IL-1b (Invivogen) reporter cells were used according to manufacturer’s protocols, as previously described.42 Briefly, binding of IL-18 to the IL-18R or IL-1b to the IL-1R induces NF-kB activation downstream, which leads to dose-dependent productions of secreted embryonic alkaline phosphatase (SEAP).

    CyQUANT Assay:

    Article Title: The tetrapeptide sequence of IL-18 and IL-1β regulates their recruitment and activation by inflammatory caspases
    Article Snippet: Other reagents used include: LPS-EB Ultrapure (Invivogen, tlrl-3pelps), VX-765 (Apexbio Technology LLC, 50-101-3604), Z-VAD-FMK (Enzo Life Sciences, NC9471015), FuGENE HD (Promega, E2311), AP20187 (Tocris 6297/5), NP-40 Lysis Buffer Low Salt (Thomas Scientific, C994H79). .. Pam3CSK4 (Invivogen, tlrl-pms), phorbol 12-myristate 12-acetate (Promega, V1171), NP-40 Lysis Buffer Low Salt (Thomas Scientific, C994H79), Recombinant Human TRAIL (R&D Systems, 375-TL/CF) Staurosporine (MedChemExpress, HY-15141), Etoposide (Enzo Life Sciences, BMC-GR307), Nigericin (Cayman Chemical, 11437), Doxycycline hydrochloride (Fisher Scientific, ICN19504401), Cyquant LDH Cytotoxicity Assay (Thermo Scientific, C20300), DC Protein Assay Kit (Bio-Rad, 5000111), HEK-Blue IL-18 (Invivogen, hkb-hmil18), HEK-Blue IL-1b (Invivogen, hkb-il1bv2), Human Total IL-18/IL-1F4 Quantikine ELISA Kit (R&D Systems, DL180), Mouse IL-1 beta/IL-1F2 Quantikine ELISA Kit (R&D Systems, MLB00C), Recombinant Human Caspase-1 (Enzo Life Sciences, BML-SE168-5000), Recombinant Human Caspase-4 (Enzo Life Sciences, BML-SE176-5000), Recombinant Human Caspase-5 (Enzo Life Sciences, BML-SE171-5000), Recombinant Mouse Caspase-11 (Enzo Life Sciences, BML-SE155-5000). .. To quantify the amount of active IL-1β and IL-18 released, HEK-Blue IL-18 and HEK-Blue IL-1β (Invivogen) reporter cells were used according to manufacturer’s protocols, as previously described.

    Article Title: The tetrapeptide sequence of IL-18 and IL-1β regulates their recruitment and activation by inflammatory caspases.
    Article Snippet: Other reagents used include: LPS-EB Ultrapure (Invivogen, tlrl-3pelps), VX-765 (Apexbio Technology LLC, 50-101-3604), Z-VAD-FMK (Enzo Life Sciences, NC9471015), FuGENE HD (Promega, E2311), AP20187 (Tocris 6297/5), NP-40 Lysis Buffer Low Salt (Thomas Scientific, C994H79). .. Pam3CSK4 (Invivogen, tlrl-pms), phorbol 12-myristate 12-acetate (Promega, V1171), NP-40 Lysis Buffer Low Salt (Thomas Scientific, C994H79), Recombinant Human TRAIL (R&D Systems, 375-TL/CF) Staurosporine (MedChemExpress, HY-15141), Etoposide (Enzo Life Sciences, BMC-GR307), Nigericin (Cayman Chemical, 11437), Doxycycline hydrochloride (Fisher Scientific, ICN19504401), Cyquant LDH Cytotoxicity Assay (Thermo Scientific, C20300), DC Protein Assay Kit (Bio-Rad, 5000111), HEK-Blue IL-18 (Invivogen, hkb-hmil18), HEK-Blue IL-1b (Invivogen, hkbil1bv2), Human Total IL-18/IL-1F4 Quantikine ELISA Kit (R&D Systems, DL180), Mouse IL-1 beta/IL-1F2 Quantikine ELISA Kit (R&D Systems, MLB00C), Recombinant Human Caspase-1 (Enzo Life Sciences, BML-SE168-5000), Recombinant Human Caspase-4 (Enzo Life Sciences, BML-SE176-5000), Recombinant Human Caspase-5 (Enzo Life Sciences, BML-SE171-5000), Recombinant Mouse Caspase-11 (Enzo Life Sciences, BML-SE155-5000). .. HEK Blue IL-1R reporter assay To quantify the amount of active IL-1b and IL-18 released, HEK-Blue IL-18 and HEK-Blue IL-1b (Invivogen) reporter cells were used according to manufacturer’s protocols, as previously described.42 Briefly, binding of IL-18 to the IL-18R or IL-1b to the IL-1R induces NF-kB activation downstream, which leads to dose-dependent productions of secreted embryonic alkaline phosphatase (SEAP).

    LDH Cytotoxicity Assay:

    Article Title: The tetrapeptide sequence of IL-18 and IL-1β regulates their recruitment and activation by inflammatory caspases
    Article Snippet: Other reagents used include: LPS-EB Ultrapure (Invivogen, tlrl-3pelps), VX-765 (Apexbio Technology LLC, 50-101-3604), Z-VAD-FMK (Enzo Life Sciences, NC9471015), FuGENE HD (Promega, E2311), AP20187 (Tocris 6297/5), NP-40 Lysis Buffer Low Salt (Thomas Scientific, C994H79). .. Pam3CSK4 (Invivogen, tlrl-pms), phorbol 12-myristate 12-acetate (Promega, V1171), NP-40 Lysis Buffer Low Salt (Thomas Scientific, C994H79), Recombinant Human TRAIL (R&D Systems, 375-TL/CF) Staurosporine (MedChemExpress, HY-15141), Etoposide (Enzo Life Sciences, BMC-GR307), Nigericin (Cayman Chemical, 11437), Doxycycline hydrochloride (Fisher Scientific, ICN19504401), Cyquant LDH Cytotoxicity Assay (Thermo Scientific, C20300), DC Protein Assay Kit (Bio-Rad, 5000111), HEK-Blue IL-18 (Invivogen, hkb-hmil18), HEK-Blue IL-1b (Invivogen, hkb-il1bv2), Human Total IL-18/IL-1F4 Quantikine ELISA Kit (R&D Systems, DL180), Mouse IL-1 beta/IL-1F2 Quantikine ELISA Kit (R&D Systems, MLB00C), Recombinant Human Caspase-1 (Enzo Life Sciences, BML-SE168-5000), Recombinant Human Caspase-4 (Enzo Life Sciences, BML-SE176-5000), Recombinant Human Caspase-5 (Enzo Life Sciences, BML-SE171-5000), Recombinant Mouse Caspase-11 (Enzo Life Sciences, BML-SE155-5000). .. To quantify the amount of active IL-1β and IL-18 released, HEK-Blue IL-18 and HEK-Blue IL-1β (Invivogen) reporter cells were used according to manufacturer’s protocols, as previously described.

    Article Title: The tetrapeptide sequence of IL-18 and IL-1β regulates their recruitment and activation by inflammatory caspases.
    Article Snippet: Other reagents used include: LPS-EB Ultrapure (Invivogen, tlrl-3pelps), VX-765 (Apexbio Technology LLC, 50-101-3604), Z-VAD-FMK (Enzo Life Sciences, NC9471015), FuGENE HD (Promega, E2311), AP20187 (Tocris 6297/5), NP-40 Lysis Buffer Low Salt (Thomas Scientific, C994H79). .. Pam3CSK4 (Invivogen, tlrl-pms), phorbol 12-myristate 12-acetate (Promega, V1171), NP-40 Lysis Buffer Low Salt (Thomas Scientific, C994H79), Recombinant Human TRAIL (R&D Systems, 375-TL/CF) Staurosporine (MedChemExpress, HY-15141), Etoposide (Enzo Life Sciences, BMC-GR307), Nigericin (Cayman Chemical, 11437), Doxycycline hydrochloride (Fisher Scientific, ICN19504401), Cyquant LDH Cytotoxicity Assay (Thermo Scientific, C20300), DC Protein Assay Kit (Bio-Rad, 5000111), HEK-Blue IL-18 (Invivogen, hkb-hmil18), HEK-Blue IL-1b (Invivogen, hkbil1bv2), Human Total IL-18/IL-1F4 Quantikine ELISA Kit (R&D Systems, DL180), Mouse IL-1 beta/IL-1F2 Quantikine ELISA Kit (R&D Systems, MLB00C), Recombinant Human Caspase-1 (Enzo Life Sciences, BML-SE168-5000), Recombinant Human Caspase-4 (Enzo Life Sciences, BML-SE176-5000), Recombinant Human Caspase-5 (Enzo Life Sciences, BML-SE171-5000), Recombinant Mouse Caspase-11 (Enzo Life Sciences, BML-SE155-5000). .. HEK Blue IL-1R reporter assay To quantify the amount of active IL-1b and IL-18 released, HEK-Blue IL-18 and HEK-Blue IL-1b (Invivogen) reporter cells were used according to manufacturer’s protocols, as previously described.42 Briefly, binding of IL-18 to the IL-18R or IL-1b to the IL-1R induces NF-kB activation downstream, which leads to dose-dependent productions of secreted embryonic alkaline phosphatase (SEAP).

    DC Protein Assay:

    Article Title: The tetrapeptide sequence of IL-18 and IL-1β regulates their recruitment and activation by inflammatory caspases
    Article Snippet: Other reagents used include: LPS-EB Ultrapure (Invivogen, tlrl-3pelps), VX-765 (Apexbio Technology LLC, 50-101-3604), Z-VAD-FMK (Enzo Life Sciences, NC9471015), FuGENE HD (Promega, E2311), AP20187 (Tocris 6297/5), NP-40 Lysis Buffer Low Salt (Thomas Scientific, C994H79). .. Pam3CSK4 (Invivogen, tlrl-pms), phorbol 12-myristate 12-acetate (Promega, V1171), NP-40 Lysis Buffer Low Salt (Thomas Scientific, C994H79), Recombinant Human TRAIL (R&D Systems, 375-TL/CF) Staurosporine (MedChemExpress, HY-15141), Etoposide (Enzo Life Sciences, BMC-GR307), Nigericin (Cayman Chemical, 11437), Doxycycline hydrochloride (Fisher Scientific, ICN19504401), Cyquant LDH Cytotoxicity Assay (Thermo Scientific, C20300), DC Protein Assay Kit (Bio-Rad, 5000111), HEK-Blue IL-18 (Invivogen, hkb-hmil18), HEK-Blue IL-1b (Invivogen, hkb-il1bv2), Human Total IL-18/IL-1F4 Quantikine ELISA Kit (R&D Systems, DL180), Mouse IL-1 beta/IL-1F2 Quantikine ELISA Kit (R&D Systems, MLB00C), Recombinant Human Caspase-1 (Enzo Life Sciences, BML-SE168-5000), Recombinant Human Caspase-4 (Enzo Life Sciences, BML-SE176-5000), Recombinant Human Caspase-5 (Enzo Life Sciences, BML-SE171-5000), Recombinant Mouse Caspase-11 (Enzo Life Sciences, BML-SE155-5000). .. To quantify the amount of active IL-1β and IL-18 released, HEK-Blue IL-18 and HEK-Blue IL-1β (Invivogen) reporter cells were used according to manufacturer’s protocols, as previously described.

    Article Title: The tetrapeptide sequence of IL-18 and IL-1β regulates their recruitment and activation by inflammatory caspases.
    Article Snippet: Other reagents used include: LPS-EB Ultrapure (Invivogen, tlrl-3pelps), VX-765 (Apexbio Technology LLC, 50-101-3604), Z-VAD-FMK (Enzo Life Sciences, NC9471015), FuGENE HD (Promega, E2311), AP20187 (Tocris 6297/5), NP-40 Lysis Buffer Low Salt (Thomas Scientific, C994H79). .. Pam3CSK4 (Invivogen, tlrl-pms), phorbol 12-myristate 12-acetate (Promega, V1171), NP-40 Lysis Buffer Low Salt (Thomas Scientific, C994H79), Recombinant Human TRAIL (R&D Systems, 375-TL/CF) Staurosporine (MedChemExpress, HY-15141), Etoposide (Enzo Life Sciences, BMC-GR307), Nigericin (Cayman Chemical, 11437), Doxycycline hydrochloride (Fisher Scientific, ICN19504401), Cyquant LDH Cytotoxicity Assay (Thermo Scientific, C20300), DC Protein Assay Kit (Bio-Rad, 5000111), HEK-Blue IL-18 (Invivogen, hkb-hmil18), HEK-Blue IL-1b (Invivogen, hkbil1bv2), Human Total IL-18/IL-1F4 Quantikine ELISA Kit (R&D Systems, DL180), Mouse IL-1 beta/IL-1F2 Quantikine ELISA Kit (R&D Systems, MLB00C), Recombinant Human Caspase-1 (Enzo Life Sciences, BML-SE168-5000), Recombinant Human Caspase-4 (Enzo Life Sciences, BML-SE176-5000), Recombinant Human Caspase-5 (Enzo Life Sciences, BML-SE171-5000), Recombinant Mouse Caspase-11 (Enzo Life Sciences, BML-SE155-5000). .. HEK Blue IL-1R reporter assay To quantify the amount of active IL-1b and IL-18 released, HEK-Blue IL-18 and HEK-Blue IL-1b (Invivogen) reporter cells were used according to manufacturer’s protocols, as previously described.42 Briefly, binding of IL-18 to the IL-18R or IL-1b to the IL-1R induces NF-kB activation downstream, which leads to dose-dependent productions of secreted embryonic alkaline phosphatase (SEAP).

    Enzyme-linked Immunosorbent Assay:

    Article Title: The tetrapeptide sequence of IL-18 and IL-1β regulates their recruitment and activation by inflammatory caspases
    Article Snippet: Other reagents used include: LPS-EB Ultrapure (Invivogen, tlrl-3pelps), VX-765 (Apexbio Technology LLC, 50-101-3604), Z-VAD-FMK (Enzo Life Sciences, NC9471015), FuGENE HD (Promega, E2311), AP20187 (Tocris 6297/5), NP-40 Lysis Buffer Low Salt (Thomas Scientific, C994H79). .. Pam3CSK4 (Invivogen, tlrl-pms), phorbol 12-myristate 12-acetate (Promega, V1171), NP-40 Lysis Buffer Low Salt (Thomas Scientific, C994H79), Recombinant Human TRAIL (R&D Systems, 375-TL/CF) Staurosporine (MedChemExpress, HY-15141), Etoposide (Enzo Life Sciences, BMC-GR307), Nigericin (Cayman Chemical, 11437), Doxycycline hydrochloride (Fisher Scientific, ICN19504401), Cyquant LDH Cytotoxicity Assay (Thermo Scientific, C20300), DC Protein Assay Kit (Bio-Rad, 5000111), HEK-Blue IL-18 (Invivogen, hkb-hmil18), HEK-Blue IL-1b (Invivogen, hkb-il1bv2), Human Total IL-18/IL-1F4 Quantikine ELISA Kit (R&D Systems, DL180), Mouse IL-1 beta/IL-1F2 Quantikine ELISA Kit (R&D Systems, MLB00C), Recombinant Human Caspase-1 (Enzo Life Sciences, BML-SE168-5000), Recombinant Human Caspase-4 (Enzo Life Sciences, BML-SE176-5000), Recombinant Human Caspase-5 (Enzo Life Sciences, BML-SE171-5000), Recombinant Mouse Caspase-11 (Enzo Life Sciences, BML-SE155-5000). .. To quantify the amount of active IL-1β and IL-18 released, HEK-Blue IL-18 and HEK-Blue IL-1β (Invivogen) reporter cells were used according to manufacturer’s protocols, as previously described.

    Article Title: The tetrapeptide sequence of IL-18 and IL-1β regulates their recruitment and activation by inflammatory caspases.
    Article Snippet: Other reagents used include: LPS-EB Ultrapure (Invivogen, tlrl-3pelps), VX-765 (Apexbio Technology LLC, 50-101-3604), Z-VAD-FMK (Enzo Life Sciences, NC9471015), FuGENE HD (Promega, E2311), AP20187 (Tocris 6297/5), NP-40 Lysis Buffer Low Salt (Thomas Scientific, C994H79). .. Pam3CSK4 (Invivogen, tlrl-pms), phorbol 12-myristate 12-acetate (Promega, V1171), NP-40 Lysis Buffer Low Salt (Thomas Scientific, C994H79), Recombinant Human TRAIL (R&D Systems, 375-TL/CF) Staurosporine (MedChemExpress, HY-15141), Etoposide (Enzo Life Sciences, BMC-GR307), Nigericin (Cayman Chemical, 11437), Doxycycline hydrochloride (Fisher Scientific, ICN19504401), Cyquant LDH Cytotoxicity Assay (Thermo Scientific, C20300), DC Protein Assay Kit (Bio-Rad, 5000111), HEK-Blue IL-18 (Invivogen, hkb-hmil18), HEK-Blue IL-1b (Invivogen, hkbil1bv2), Human Total IL-18/IL-1F4 Quantikine ELISA Kit (R&D Systems, DL180), Mouse IL-1 beta/IL-1F2 Quantikine ELISA Kit (R&D Systems, MLB00C), Recombinant Human Caspase-1 (Enzo Life Sciences, BML-SE168-5000), Recombinant Human Caspase-4 (Enzo Life Sciences, BML-SE176-5000), Recombinant Human Caspase-5 (Enzo Life Sciences, BML-SE171-5000), Recombinant Mouse Caspase-11 (Enzo Life Sciences, BML-SE155-5000). .. HEK Blue IL-1R reporter assay To quantify the amount of active IL-1b and IL-18 released, HEK-Blue IL-18 and HEK-Blue IL-1b (Invivogen) reporter cells were used according to manufacturer’s protocols, as previously described.42 Briefly, binding of IL-18 to the IL-18R or IL-1b to the IL-1R induces NF-kB activation downstream, which leads to dose-dependent productions of secreted embryonic alkaline phosphatase (SEAP).

    other:

    Article Title: The tetrapeptide sequence of IL-18 and IL-1β regulates their recruitment and activation by inflammatory caspases.
    Article Snippet: HEK Blue IL-1R reporter assay To quantify the amount of active IL-1b and IL-18 released, HEK-Blue IL-18 and HEK-Blue IL-1b (Invivogen) reporter cells were used according to manufacturer’s protocols, as previously described.42 Briefly, binding of IL-18 to the IL-18R or IL-1b to the IL-1R induces NF-kB activation downstream, which leads to dose-dependent productions of secreted embryonic alkaline phosphatase (SEAP).

    Cell Stimulation:

    Article Title:
    Article Snippet: .. Recombinant human TRAIL was purchased from R&D System, Inc (Minneapolis, MN), and was used for cell stimulation in all experiments at a concentration of 20 ng/ml or as indicated in the figure legends. ..

    Concentration Assay:

    Article Title:
    Article Snippet: .. Recombinant human TRAIL was purchased from R&D System, Inc (Minneapolis, MN), and was used for cell stimulation in all experiments at a concentration of 20 ng/ml or as indicated in the figure legends. ..



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