Recombinant:
Article Title: The tetrapeptide sequence of IL-18 and IL-1β regulates their recruitment and activation by inflammatory caspases
Article Snippet: Other reagents used include: LPS-EB Ultrapure (Invivogen, tlrl-3pelps), VX-765 (Apexbio Technology LLC, 50-101-3604), Z-VAD-FMK (Enzo Life Sciences, NC9471015), FuGENE HD (Promega, E2311), AP20187 (Tocris 6297/5), NP-40 Lysis Buffer Low Salt (Thomas Scientific, C994H79). .. Pam3CSK4 (Invivogen, tlrl-pms), phorbol 12-myristate 12-acetate (Promega, V1171), NP-40 Lysis Buffer Low Salt (Thomas Scientific, C994H79), Recombinant Human TRAIL (R&D Systems, 375-TL/CF) Staurosporine (MedChemExpress, HY-15141), Etoposide (Enzo Life Sciences, BMC-GR307), Nigericin (Cayman Chemical, 11437), Doxycycline hydrochloride (Fisher Scientific, ICN19504401), Cyquant LDH Cytotoxicity Assay (Thermo Scientific, C20300), DC Protein Assay Kit (Bio-Rad, 5000111), HEK-Blue IL-18 (Invivogen, hkb-hmil18), HEK-Blue IL-1b (Invivogen, hkb-il1bv2), Human Total IL-18/IL-1F4 Quantikine ELISA Kit (R&D Systems, DL180), Mouse IL-1 beta/IL-1F2 Quantikine ELISA Kit (R&D Systems, MLB00C), Recombinant Human Caspase-1 (Enzo Life Sciences, BML-SE168-5000), Recombinant Human Caspase-4 (Enzo Life Sciences, BML-SE176-5000), Recombinant Human Caspase-5 (Enzo Life Sciences, BML-SE171-5000), Recombinant Mouse Caspase-11 (Enzo Life Sciences, BML-SE155-5000). .. To quantify the amount of active IL-1β and IL-18 released, HEK-Blue IL-18 and HEK-Blue IL-1β (Invivogen) reporter cells were used according to manufacturer’s protocols, as previously described.
Article Title: The tetrapeptide sequence of IL-18 and IL-1β regulates their recruitment and activation by inflammatory caspases.
Article Snippet: Other reagents used include: LPS-EB Ultrapure (Invivogen, tlrl-3pelps), VX-765 (Apexbio Technology LLC, 50-101-3604), Z-VAD-FMK (Enzo Life Sciences, NC9471015), FuGENE HD (Promega, E2311), AP20187 (Tocris 6297/5), NP-40 Lysis Buffer Low Salt (Thomas Scientific, C994H79). .. Pam3CSK4 (Invivogen, tlrl-pms), phorbol 12-myristate 12-acetate (Promega, V1171), NP-40 Lysis Buffer Low Salt (Thomas Scientific, C994H79), Recombinant Human TRAIL (R&D Systems, 375-TL/CF) Staurosporine (MedChemExpress, HY-15141), Etoposide (Enzo Life Sciences, BMC-GR307), Nigericin (Cayman Chemical, 11437), Doxycycline hydrochloride (Fisher Scientific, ICN19504401), Cyquant LDH Cytotoxicity Assay (Thermo Scientific, C20300), DC Protein Assay Kit (Bio-Rad, 5000111), HEK-Blue IL-18 (Invivogen, hkb-hmil18), HEK-Blue IL-1b (Invivogen, hkbil1bv2), Human Total IL-18/IL-1F4 Quantikine ELISA Kit (R&D Systems, DL180), Mouse IL-1 beta/IL-1F2 Quantikine ELISA Kit (R&D Systems, MLB00C), Recombinant Human Caspase-1 (Enzo Life Sciences, BML-SE168-5000), Recombinant Human Caspase-4 (Enzo Life Sciences, BML-SE176-5000), Recombinant Human Caspase-5 (Enzo Life Sciences, BML-SE171-5000), Recombinant Mouse Caspase-11 (Enzo Life Sciences, BML-SE155-5000). .. HEK Blue IL-1R reporter assay To quantify the amount of active IL-1b and IL-18 released, HEK-Blue IL-18 and HEK-Blue IL-1b (Invivogen) reporter cells were used according to manufacturer’s protocols, as previously described.42 Briefly, binding of IL-18 to the IL-18R or IL-1b to the IL-1R induces NF-kB activation downstream, which leads to dose-dependent productions of secreted embryonic alkaline phosphatase (SEAP).
Article Title:
Article Snippet: .. Recombinant human TRAIL was purchased from R&D System, Inc (Minneapolis, MN), and was used for cell stimulation in all experiments at a concentration of 20 ng/ml or as indicated in the figure legends. ..
Incubation:
Lysis:Article Title: The tetrapeptide sequence of IL-18 and IL-1β regulates their recruitment and activation by inflammatory caspases
Article Snippet: Other reagents used include: LPS-EB Ultrapure (Invivogen, tlrl-3pelps), VX-765 (Apexbio Technology LLC, 50-101-3604), Z-VAD-FMK (Enzo Life Sciences, NC9471015), FuGENE HD (Promega, E2311), AP20187 (Tocris 6297/5), NP-40 Lysis Buffer Low Salt (Thomas Scientific, C994H79). .. Pam3CSK4 (Invivogen, tlrl-pms), phorbol 12-myristate 12-acetate (Promega, V1171), NP-40 Lysis Buffer Low Salt (Thomas Scientific, C994H79), Recombinant Human TRAIL (R&D Systems, 375-TL/CF) Staurosporine (MedChemExpress, HY-15141), Etoposide (Enzo Life Sciences, BMC-GR307), Nigericin (Cayman Chemical, 11437), Doxycycline hydrochloride (Fisher Scientific, ICN19504401), Cyquant LDH Cytotoxicity Assay (Thermo Scientific, C20300), DC Protein Assay Kit (Bio-Rad, 5000111), HEK-Blue IL-18 (Invivogen, hkb-hmil18), HEK-Blue IL-1b (Invivogen, hkb-il1bv2), Human Total IL-18/IL-1F4 Quantikine ELISA Kit (R&D Systems, DL180), Mouse IL-1 beta/IL-1F2 Quantikine ELISA Kit (R&D Systems, MLB00C), Recombinant Human Caspase-1 (Enzo Life Sciences, BML-SE168-5000), Recombinant Human Caspase-4 (Enzo Life Sciences, BML-SE176-5000), Recombinant Human Caspase-5 (Enzo Life Sciences, BML-SE171-5000), Recombinant Mouse Caspase-11 (Enzo Life Sciences, BML-SE155-5000). .. To quantify the amount of active IL-1β and IL-18 released, HEK-Blue IL-18 and HEK-Blue IL-1β (Invivogen) reporter cells were used according to manufacturer’s protocols, as previously described.
Article Title: The tetrapeptide sequence of IL-18 and IL-1β regulates their recruitment and activation by inflammatory caspases.
Article Snippet: Other reagents used include: LPS-EB Ultrapure (Invivogen, tlrl-3pelps), VX-765 (Apexbio Technology LLC, 50-101-3604), Z-VAD-FMK (Enzo Life Sciences, NC9471015), FuGENE HD (Promega, E2311), AP20187 (Tocris 6297/5), NP-40 Lysis Buffer Low Salt (Thomas Scientific, C994H79). .. Pam3CSK4 (Invivogen, tlrl-pms), phorbol 12-myristate 12-acetate (Promega, V1171), NP-40 Lysis Buffer Low Salt (Thomas Scientific, C994H79), Recombinant Human TRAIL (R&D Systems, 375-TL/CF) Staurosporine (MedChemExpress, HY-15141), Etoposide (Enzo Life Sciences, BMC-GR307), Nigericin (Cayman Chemical, 11437), Doxycycline hydrochloride (Fisher Scientific, ICN19504401), Cyquant LDH Cytotoxicity Assay (Thermo Scientific, C20300), DC Protein Assay Kit (Bio-Rad, 5000111), HEK-Blue IL-18 (Invivogen, hkb-hmil18), HEK-Blue IL-1b (Invivogen, hkbil1bv2), Human Total IL-18/IL-1F4 Quantikine ELISA Kit (R&D Systems, DL180), Mouse IL-1 beta/IL-1F2 Quantikine ELISA Kit (R&D Systems, MLB00C), Recombinant Human Caspase-1 (Enzo Life Sciences, BML-SE168-5000), Recombinant Human Caspase-4 (Enzo Life Sciences, BML-SE176-5000), Recombinant Human Caspase-5 (Enzo Life Sciences, BML-SE171-5000), Recombinant Mouse Caspase-11 (Enzo Life Sciences, BML-SE155-5000). .. HEK Blue IL-1R reporter assay To quantify the amount of active IL-1b and IL-18 released, HEK-Blue IL-18 and HEK-Blue IL-1b (Invivogen) reporter cells were used according to manufacturer’s protocols, as previously described.42 Briefly, binding of IL-18 to the IL-18R or IL-1b to the IL-1R induces NF-kB activation downstream, which leads to dose-dependent productions of secreted embryonic alkaline phosphatase (SEAP).
CyQUANT Assay:Article Title: The tetrapeptide sequence of IL-18 and IL-1β regulates their recruitment and activation by inflammatory caspases
Article Snippet: Other reagents used include: LPS-EB Ultrapure (Invivogen, tlrl-3pelps), VX-765 (Apexbio Technology LLC, 50-101-3604), Z-VAD-FMK (Enzo Life Sciences, NC9471015), FuGENE HD (Promega, E2311), AP20187 (Tocris 6297/5), NP-40 Lysis Buffer Low Salt (Thomas Scientific, C994H79). .. Pam3CSK4 (Invivogen, tlrl-pms), phorbol 12-myristate 12-acetate (Promega, V1171), NP-40 Lysis Buffer Low Salt (Thomas Scientific, C994H79), Recombinant Human TRAIL (R&D Systems, 375-TL/CF) Staurosporine (MedChemExpress, HY-15141), Etoposide (Enzo Life Sciences, BMC-GR307), Nigericin (Cayman Chemical, 11437), Doxycycline hydrochloride (Fisher Scientific, ICN19504401), Cyquant LDH Cytotoxicity Assay (Thermo Scientific, C20300), DC Protein Assay Kit (Bio-Rad, 5000111), HEK-Blue IL-18 (Invivogen, hkb-hmil18), HEK-Blue IL-1b (Invivogen, hkb-il1bv2), Human Total IL-18/IL-1F4 Quantikine ELISA Kit (R&D Systems, DL180), Mouse IL-1 beta/IL-1F2 Quantikine ELISA Kit (R&D Systems, MLB00C), Recombinant Human Caspase-1 (Enzo Life Sciences, BML-SE168-5000), Recombinant Human Caspase-4 (Enzo Life Sciences, BML-SE176-5000), Recombinant Human Caspase-5 (Enzo Life Sciences, BML-SE171-5000), Recombinant Mouse Caspase-11 (Enzo Life Sciences, BML-SE155-5000). .. To quantify the amount of active IL-1β and IL-18 released, HEK-Blue IL-18 and HEK-Blue IL-1β (Invivogen) reporter cells were used according to manufacturer’s protocols, as previously described.
Article Title: The tetrapeptide sequence of IL-18 and IL-1β regulates their recruitment and activation by inflammatory caspases.
Article Snippet: Other reagents used include: LPS-EB Ultrapure (Invivogen, tlrl-3pelps), VX-765 (Apexbio Technology LLC, 50-101-3604), Z-VAD-FMK (Enzo Life Sciences, NC9471015), FuGENE HD (Promega, E2311), AP20187 (Tocris 6297/5), NP-40 Lysis Buffer Low Salt (Thomas Scientific, C994H79). .. Pam3CSK4 (Invivogen, tlrl-pms), phorbol 12-myristate 12-acetate (Promega, V1171), NP-40 Lysis Buffer Low Salt (Thomas Scientific, C994H79), Recombinant Human TRAIL (R&D Systems, 375-TL/CF) Staurosporine (MedChemExpress, HY-15141), Etoposide (Enzo Life Sciences, BMC-GR307), Nigericin (Cayman Chemical, 11437), Doxycycline hydrochloride (Fisher Scientific, ICN19504401), Cyquant LDH Cytotoxicity Assay (Thermo Scientific, C20300), DC Protein Assay Kit (Bio-Rad, 5000111), HEK-Blue IL-18 (Invivogen, hkb-hmil18), HEK-Blue IL-1b (Invivogen, hkbil1bv2), Human Total IL-18/IL-1F4 Quantikine ELISA Kit (R&D Systems, DL180), Mouse IL-1 beta/IL-1F2 Quantikine ELISA Kit (R&D Systems, MLB00C), Recombinant Human Caspase-1 (Enzo Life Sciences, BML-SE168-5000), Recombinant Human Caspase-4 (Enzo Life Sciences, BML-SE176-5000), Recombinant Human Caspase-5 (Enzo Life Sciences, BML-SE171-5000), Recombinant Mouse Caspase-11 (Enzo Life Sciences, BML-SE155-5000). .. HEK Blue IL-1R reporter assay To quantify the amount of active IL-1b and IL-18 released, HEK-Blue IL-18 and HEK-Blue IL-1b (Invivogen) reporter cells were used according to manufacturer’s protocols, as previously described.42 Briefly, binding of IL-18 to the IL-18R or IL-1b to the IL-1R induces NF-kB activation downstream, which leads to dose-dependent productions of secreted embryonic alkaline phosphatase (SEAP).
LDH Cytotoxicity Assay:Article Title: The tetrapeptide sequence of IL-18 and IL-1β regulates their recruitment and activation by inflammatory caspases
Article Snippet: Other reagents used include: LPS-EB Ultrapure (Invivogen, tlrl-3pelps), VX-765 (Apexbio Technology LLC, 50-101-3604), Z-VAD-FMK (Enzo Life Sciences, NC9471015), FuGENE HD (Promega, E2311), AP20187 (Tocris 6297/5), NP-40 Lysis Buffer Low Salt (Thomas Scientific, C994H79). .. Pam3CSK4 (Invivogen, tlrl-pms), phorbol 12-myristate 12-acetate (Promega, V1171), NP-40 Lysis Buffer Low Salt (Thomas Scientific, C994H79), Recombinant Human TRAIL (R&D Systems, 375-TL/CF) Staurosporine (MedChemExpress, HY-15141), Etoposide (Enzo Life Sciences, BMC-GR307), Nigericin (Cayman Chemical, 11437), Doxycycline hydrochloride (Fisher Scientific, ICN19504401), Cyquant LDH Cytotoxicity Assay (Thermo Scientific, C20300), DC Protein Assay Kit (Bio-Rad, 5000111), HEK-Blue IL-18 (Invivogen, hkb-hmil18), HEK-Blue IL-1b (Invivogen, hkb-il1bv2), Human Total IL-18/IL-1F4 Quantikine ELISA Kit (R&D Systems, DL180), Mouse IL-1 beta/IL-1F2 Quantikine ELISA Kit (R&D Systems, MLB00C), Recombinant Human Caspase-1 (Enzo Life Sciences, BML-SE168-5000), Recombinant Human Caspase-4 (Enzo Life Sciences, BML-SE176-5000), Recombinant Human Caspase-5 (Enzo Life Sciences, BML-SE171-5000), Recombinant Mouse Caspase-11 (Enzo Life Sciences, BML-SE155-5000). .. To quantify the amount of active IL-1β and IL-18 released, HEK-Blue IL-18 and HEK-Blue IL-1β (Invivogen) reporter cells were used according to manufacturer’s protocols, as previously described.
Article Title: The tetrapeptide sequence of IL-18 and IL-1β regulates their recruitment and activation by inflammatory caspases.
Article Snippet: Other reagents used include: LPS-EB Ultrapure (Invivogen, tlrl-3pelps), VX-765 (Apexbio Technology LLC, 50-101-3604), Z-VAD-FMK (Enzo Life Sciences, NC9471015), FuGENE HD (Promega, E2311), AP20187 (Tocris 6297/5), NP-40 Lysis Buffer Low Salt (Thomas Scientific, C994H79). .. Pam3CSK4 (Invivogen, tlrl-pms), phorbol 12-myristate 12-acetate (Promega, V1171), NP-40 Lysis Buffer Low Salt (Thomas Scientific, C994H79), Recombinant Human TRAIL (R&D Systems, 375-TL/CF) Staurosporine (MedChemExpress, HY-15141), Etoposide (Enzo Life Sciences, BMC-GR307), Nigericin (Cayman Chemical, 11437), Doxycycline hydrochloride (Fisher Scientific, ICN19504401), Cyquant LDH Cytotoxicity Assay (Thermo Scientific, C20300), DC Protein Assay Kit (Bio-Rad, 5000111), HEK-Blue IL-18 (Invivogen, hkb-hmil18), HEK-Blue IL-1b (Invivogen, hkbil1bv2), Human Total IL-18/IL-1F4 Quantikine ELISA Kit (R&D Systems, DL180), Mouse IL-1 beta/IL-1F2 Quantikine ELISA Kit (R&D Systems, MLB00C), Recombinant Human Caspase-1 (Enzo Life Sciences, BML-SE168-5000), Recombinant Human Caspase-4 (Enzo Life Sciences, BML-SE176-5000), Recombinant Human Caspase-5 (Enzo Life Sciences, BML-SE171-5000), Recombinant Mouse Caspase-11 (Enzo Life Sciences, BML-SE155-5000). .. HEK Blue IL-1R reporter assay To quantify the amount of active IL-1b and IL-18 released, HEK-Blue IL-18 and HEK-Blue IL-1b (Invivogen) reporter cells were used according to manufacturer’s protocols, as previously described.42 Briefly, binding of IL-18 to the IL-18R or IL-1b to the IL-1R induces NF-kB activation downstream, which leads to dose-dependent productions of secreted embryonic alkaline phosphatase (SEAP).
DC Protein Assay:Article Title: The tetrapeptide sequence of IL-18 and IL-1β regulates their recruitment and activation by inflammatory caspases
Article Snippet: Other reagents used include: LPS-EB Ultrapure (Invivogen, tlrl-3pelps), VX-765 (Apexbio Technology LLC, 50-101-3604), Z-VAD-FMK (Enzo Life Sciences, NC9471015), FuGENE HD (Promega, E2311), AP20187 (Tocris 6297/5), NP-40 Lysis Buffer Low Salt (Thomas Scientific, C994H79). .. Pam3CSK4 (Invivogen, tlrl-pms), phorbol 12-myristate 12-acetate (Promega, V1171), NP-40 Lysis Buffer Low Salt (Thomas Scientific, C994H79), Recombinant Human TRAIL (R&D Systems, 375-TL/CF) Staurosporine (MedChemExpress, HY-15141), Etoposide (Enzo Life Sciences, BMC-GR307), Nigericin (Cayman Chemical, 11437), Doxycycline hydrochloride (Fisher Scientific, ICN19504401), Cyquant LDH Cytotoxicity Assay (Thermo Scientific, C20300), DC Protein Assay Kit (Bio-Rad, 5000111), HEK-Blue IL-18 (Invivogen, hkb-hmil18), HEK-Blue IL-1b (Invivogen, hkb-il1bv2), Human Total IL-18/IL-1F4 Quantikine ELISA Kit (R&D Systems, DL180), Mouse IL-1 beta/IL-1F2 Quantikine ELISA Kit (R&D Systems, MLB00C), Recombinant Human Caspase-1 (Enzo Life Sciences, BML-SE168-5000), Recombinant Human Caspase-4 (Enzo Life Sciences, BML-SE176-5000), Recombinant Human Caspase-5 (Enzo Life Sciences, BML-SE171-5000), Recombinant Mouse Caspase-11 (Enzo Life Sciences, BML-SE155-5000). .. To quantify the amount of active IL-1β and IL-18 released, HEK-Blue IL-18 and HEK-Blue IL-1β (Invivogen) reporter cells were used according to manufacturer’s protocols, as previously described.
Article Title: The tetrapeptide sequence of IL-18 and IL-1β regulates their recruitment and activation by inflammatory caspases.
Article Snippet: Other reagents used include: LPS-EB Ultrapure (Invivogen, tlrl-3pelps), VX-765 (Apexbio Technology LLC, 50-101-3604), Z-VAD-FMK (Enzo Life Sciences, NC9471015), FuGENE HD (Promega, E2311), AP20187 (Tocris 6297/5), NP-40 Lysis Buffer Low Salt (Thomas Scientific, C994H79). .. Pam3CSK4 (Invivogen, tlrl-pms), phorbol 12-myristate 12-acetate (Promega, V1171), NP-40 Lysis Buffer Low Salt (Thomas Scientific, C994H79), Recombinant Human TRAIL (R&D Systems, 375-TL/CF) Staurosporine (MedChemExpress, HY-15141), Etoposide (Enzo Life Sciences, BMC-GR307), Nigericin (Cayman Chemical, 11437), Doxycycline hydrochloride (Fisher Scientific, ICN19504401), Cyquant LDH Cytotoxicity Assay (Thermo Scientific, C20300), DC Protein Assay Kit (Bio-Rad, 5000111), HEK-Blue IL-18 (Invivogen, hkb-hmil18), HEK-Blue IL-1b (Invivogen, hkbil1bv2), Human Total IL-18/IL-1F4 Quantikine ELISA Kit (R&D Systems, DL180), Mouse IL-1 beta/IL-1F2 Quantikine ELISA Kit (R&D Systems, MLB00C), Recombinant Human Caspase-1 (Enzo Life Sciences, BML-SE168-5000), Recombinant Human Caspase-4 (Enzo Life Sciences, BML-SE176-5000), Recombinant Human Caspase-5 (Enzo Life Sciences, BML-SE171-5000), Recombinant Mouse Caspase-11 (Enzo Life Sciences, BML-SE155-5000). .. HEK Blue IL-1R reporter assay To quantify the amount of active IL-1b and IL-18 released, HEK-Blue IL-18 and HEK-Blue IL-1b (Invivogen) reporter cells were used according to manufacturer’s protocols, as previously described.42 Briefly, binding of IL-18 to the IL-18R or IL-1b to the IL-1R induces NF-kB activation downstream, which leads to dose-dependent productions of secreted embryonic alkaline phosphatase (SEAP).
Enzyme-linked Immunosorbent Assay:Article Title: The tetrapeptide sequence of IL-18 and IL-1β regulates their recruitment and activation by inflammatory caspases
Article Snippet: Other reagents used include: LPS-EB Ultrapure (Invivogen, tlrl-3pelps), VX-765 (Apexbio Technology LLC, 50-101-3604), Z-VAD-FMK (Enzo Life Sciences, NC9471015), FuGENE HD (Promega, E2311), AP20187 (Tocris 6297/5), NP-40 Lysis Buffer Low Salt (Thomas Scientific, C994H79). .. Pam3CSK4 (Invivogen, tlrl-pms), phorbol 12-myristate 12-acetate (Promega, V1171), NP-40 Lysis Buffer Low Salt (Thomas Scientific, C994H79), Recombinant Human TRAIL (R&D Systems, 375-TL/CF) Staurosporine (MedChemExpress, HY-15141), Etoposide (Enzo Life Sciences, BMC-GR307), Nigericin (Cayman Chemical, 11437), Doxycycline hydrochloride (Fisher Scientific, ICN19504401), Cyquant LDH Cytotoxicity Assay (Thermo Scientific, C20300), DC Protein Assay Kit (Bio-Rad, 5000111), HEK-Blue IL-18 (Invivogen, hkb-hmil18), HEK-Blue IL-1b (Invivogen, hkb-il1bv2), Human Total IL-18/IL-1F4 Quantikine ELISA Kit (R&D Systems, DL180), Mouse IL-1 beta/IL-1F2 Quantikine ELISA Kit (R&D Systems, MLB00C), Recombinant Human Caspase-1 (Enzo Life Sciences, BML-SE168-5000), Recombinant Human Caspase-4 (Enzo Life Sciences, BML-SE176-5000), Recombinant Human Caspase-5 (Enzo Life Sciences, BML-SE171-5000), Recombinant Mouse Caspase-11 (Enzo Life Sciences, BML-SE155-5000). .. To quantify the amount of active IL-1β and IL-18 released, HEK-Blue IL-18 and HEK-Blue IL-1β (Invivogen) reporter cells were used according to manufacturer’s protocols, as previously described.
Article Title: The tetrapeptide sequence of IL-18 and IL-1β regulates their recruitment and activation by inflammatory caspases.
Article Snippet: Other reagents used include: LPS-EB Ultrapure (Invivogen, tlrl-3pelps), VX-765 (Apexbio Technology LLC, 50-101-3604), Z-VAD-FMK (Enzo Life Sciences, NC9471015), FuGENE HD (Promega, E2311), AP20187 (Tocris 6297/5), NP-40 Lysis Buffer Low Salt (Thomas Scientific, C994H79). .. Pam3CSK4 (Invivogen, tlrl-pms), phorbol 12-myristate 12-acetate (Promega, V1171), NP-40 Lysis Buffer Low Salt (Thomas Scientific, C994H79), Recombinant Human TRAIL (R&D Systems, 375-TL/CF) Staurosporine (MedChemExpress, HY-15141), Etoposide (Enzo Life Sciences, BMC-GR307), Nigericin (Cayman Chemical, 11437), Doxycycline hydrochloride (Fisher Scientific, ICN19504401), Cyquant LDH Cytotoxicity Assay (Thermo Scientific, C20300), DC Protein Assay Kit (Bio-Rad, 5000111), HEK-Blue IL-18 (Invivogen, hkb-hmil18), HEK-Blue IL-1b (Invivogen, hkbil1bv2), Human Total IL-18/IL-1F4 Quantikine ELISA Kit (R&D Systems, DL180), Mouse IL-1 beta/IL-1F2 Quantikine ELISA Kit (R&D Systems, MLB00C), Recombinant Human Caspase-1 (Enzo Life Sciences, BML-SE168-5000), Recombinant Human Caspase-4 (Enzo Life Sciences, BML-SE176-5000), Recombinant Human Caspase-5 (Enzo Life Sciences, BML-SE171-5000), Recombinant Mouse Caspase-11 (Enzo Life Sciences, BML-SE155-5000). .. HEK Blue IL-1R reporter assay To quantify the amount of active IL-1b and IL-18 released, HEK-Blue IL-18 and HEK-Blue IL-1b (Invivogen) reporter cells were used according to manufacturer’s protocols, as previously described.42 Briefly, binding of IL-18 to the IL-18R or IL-1b to the IL-1R induces NF-kB activation downstream, which leads to dose-dependent productions of secreted embryonic alkaline phosphatase (SEAP).
other:
Cell Stimulation:Article Title:
Article Snippet: .. Recombinant human TRAIL was purchased from R&D System, Inc (Minneapolis, MN), and was used for cell stimulation in all experiments at a concentration of 20 ng/ml or as indicated in the figure legends. ..
Concentration Assay:Article Title:
Article Snippet: .. Recombinant human TRAIL was purchased from R&D System, Inc (Minneapolis, MN), and was used for cell stimulation in all experiments at a concentration of 20 ng/ml or as indicated in the figure legends. ..
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